Vipin Paliwal, Ph.D.
Associate Professor
- Milwaukee WI UNITED STATES
- Allen Bradley Hall of Science: S203
- Physics and Chemistry
Expertise in the field of Biomedical research, with a foucs on Biochemistry and Immunology.
Multimedia
Education, Licensure and Certification
Ph.D.
Biochemistry
Post Graduate Medical Institute
1985
M.S.
Biochemistry
Kurukshetra University
1981
B.S.
Chemistry
Guru Nanak Dev University
1978
Biography
Areas of Expertise
Accomplishments
MSOE Professional Development Grant. Awarded to Nazieh Masoud and Vipin Paliwal, Department of Physics and Chemistry
2018
MSOE Nominated for Karl O. Werwath Research Award
2006, 2013, 2014, 2015
MSOE Protracted Leave Award for Biomedical Research
2007
Affiliations
- American Society for Biochemistry and Molecular Biochemistry (ASBMB)
Languages
- Hindi
Event and Speaking Appearances
Acetaminophen toxicity in Two and Three-Dimensional Rat Hepatocyte Cultures
Annual American Society for Biochemistry & Molecular Biology Meeting Chicago, IL
2017-04-01
Three-dimensional Cell Culture of Rat Hepatocytes in Sub-microliter Hydrogel Wells
American Society for Biochemistry & Molecular Biology Meeting Boston MA
2015-03-27
Development of a portable UV light source to be used in Chemistry classes and for research activities
Fall Forum Presentations, MSOE
2018-09-19
Creation of tPA Conjugated Dendrimer Nanoparticles
Association of Biochemistry & Molecular Biology (ASBMB) Annual Meeting San Diego
2014-04-25
Creation of a Physical 3D Homo Sapien Single-cell Model Using Confocal Microscopy Data Via Rapid Prototyping
Association of Biochemistry & Molecular Biology (ASBMB) Annual Meeting Boston, MA
2013-04-02
Teaching Areas
Organic Chemistry
Organic Chemistry I with laboratory, Organic Chemistry II with laboratory
Nursing Chemistry
Teach General, organic and Biochemistry sequence for life sciences.
Research Interests
Skin Cancer reserach
Skin cancer is one of the most common forms of cancer in US. Prolonged exposure to sunlight is one of the major causes of skin cancer. UV-B radiations in the region of 290-320 nm of the solar spectrum are absorbed by skin cells resulting in mutated DNA leading to cancer. I am interested in studying formation of 6-4 pyrimidine-pyrimidone photoproducts following UVB exposure.
Active learning activity on skin cancer & DNA mutations
I am interested in developing classroom activities for teaching DNA mutations and protection of skin cancer by popular commercial sunscreens.
Selected Publications
A hands-on learning activity on potential DNA damage and skin cancer for a nursing biochemistry class
American Society for Biochemistry & Molecular Biology (ASBMB) e-poster,Vipin Paliwal, Nazieh Masoud and Anne-Marie Nickel
May 28, 2020
In this activity, the relative intensity of UV radiation at 308 nm in the absence and presence of sunscreen was measured by undergraduate first year nursing biochemistry students at the Milwaukee School of Engineering (MSOE). A class of 20 students brought in their own commercially available sunscreen to class. Students tested the sunscreens’ ability to block the UVB radiations. Students made connections between the real-life classroom activity performed and a three-dimensional physical model of DNA segment (crystal structure source entries: ndb UD0053 or PDB 1SM5) showing the formation of the T-T dimer using a hand-held model and images.
Collaboration Synergy, Progression, and Perseverance: Keys to Successful Undergraduate Research
ASME 2017 International Mechanical Engineering Congress and ExpositionKumpaty, S., Paliwal, V. and Parrish, T.
2017
The primary author has been the principal investigator of the Milwaukee School of Engineering’s Research Experiences for Undergraduates program, sponsored by the National Science Foundation for the past twenty years. Reflecting on the most recent projects, the authors present some keys to success at the undergraduate level, especially with the limitation of only a 10-week duration for the summer research enterprise. With a clear designation of an individual project to be passionately owned by a participant, ensuring success by each participant warrants many ingredients to be in place and the cooperation of various constituents at the right time and pace. In the grand scheme of this research experience, the participant develops and grows, bringing the wholeness to the purpose for which the program exists. Some fascinating observations of the summer undergraduate research program are presented as key ingredients to the success. These include but are not limited to collaboration, synergy, progression and perseverance.
A subset of AID-dependent B-1a cells initiates hypersensitivity and pneumococcal pneumonia resistance
Annals of the New York Academy of SciencesAskenase, P.W., Bryniarski, K., Paliwal, V., Redegeld, F., Kormelink, T.G., Kerfoot, S., Hutchinson, A.T., van Loveren, H., Campos, R., Itakura, A., Majewska-Szczepanik, M.
2015
We propose that there is a special B-1a B cell subset (“sB-1a” cells) that mediates linked processes very early after immunization to initiate cutaneous contact sensitivity (CS), delayed-type hypersensitivity (DTH), and immune resistance to pneumococcal pneumonia. Our published data indicate that in CS and DTH, these initiating processes are required for elicitation of the delayed onset and late-occurring classical T cell–mediated responses. sB-1a cells resemble memory B2 cells, as they are stimulated within 1 h of immunization and depend on T helper cytokines—uniquely IL-4 from hepatic iNKT cells—for activation and rapid migration from the peritoneal cavity to the spleen to secrete IgM antibody (Ab) and Ab-derived free light chains (FLCs) by only 1 day after immunization. Unlike conventional B-1a (cB-1a) cell–produced IgM natural Ab, IgM Ab produced by sB-1a cells has high Ag affinity owing to immunoglobulin V-region mutations induced by activation-induced cytidine deaminase (AID). The dominant cB-1a cells are increased in immunized AID-deficient mice but do not mediate initiation, CS, or pneumonia resistance because natural Ab has relatively low Ag affinity because of unmutated germ-line V regions. In CS and DTH, sB-1a IgM Ag affinity is sufficiently high to mediate complement activation for generation of C5a that, together with vasoactive mediators such as TNF-α released by FLC-sensitized mast cells, activate local endothelium for extravascular recruitment of effector T cells. We conclude by discussing the possibility of functional sB-1 cells in humans.
An inventory of traditional herbal medicines used in management of diabetes mellitus II by ethnic people of south-east Rajasthan (India)
International Journal of Pharmaceutical Sciences Review and ResearchArora, A., Paliwal, V., Jain, H.
2015
Stress leads to disease and disease leads to stress, this bidirectional poking relationship urges for the development of stress busters or ailment regulators which can sooth lives. Ethnic groups of south east Rajasthan inherit a vast pharmacopoeia and ingredient/s sourced from the same, forms a baseline of numerable commercial drugs. Present study was an attempt to inventorize the hypoglycemic plants and their formulations used by tribals to regulate the blood glucose levels. Crisscross transactional interaction with local healers and ethnic groups of both gender and various age groups reveal use of 41 plants and 8 formulations for DM II management. Among documented ethno phyto drugs 8 were used as food while 16 as protective source to delay the onset of DM II.34 plants were used as curative agents i.e. to regulate the glucose levels. Enlisted data includes mode of usage, usage time tenure, quantity and use value (UV) of documented ethno hypoglycemic plants.
Vitamin B Complex and Bioheat Transfer Projects: 2008 Summer Research Experiences for Teachers at Milwaukee School of Engineering
ASME International Mechanical Engineering Congress and ExpositionKumpaty, S., Foster, A., Hutson, A., Paliwal, V.
2009
This paper describes the summer research experiences of a high-school chemistry and biology teacher and a middle-school science teacher at the Milwaukee School of Engineering (MSOE). The first project involved researching B Complex Vitamins at MSOE’s Center for Biomolecular Modeling, developing molecular models using rapid prototyping technology (Z Corp 3D Printer) and creating curriculum modules for teaching the role of B Complex Vitamins to chemistry students in public high schools. A Javascript/HTML for interactive and dynamic presentation for understanding of thiamine (Vitamin B1) via web was written and implemented with Jmol software. A visual inspection of the family of Vitamin B Complex created and the curriculum modules developed during this project provide exciting and effective learning avenues for students in biology and chemistry classrooms. The second project dealt with the study of bioheat transfer and its simulation using MATLAB, and creation of a curriculum module that illustrates heat transfer principles reinforced by implementing the simulation. The teachers enjoyed the interaction with their advisors and the positive environment for their professional development. Details of their projects and experiences along with the evaluation of the program are presented in this paper. The teachers were pleased to be involved in connecting physics, biology, engineering and math into real projects that will motivate the students in their classes to pursue careers in STEM fields.
An Hour after Immunization Peritoneal B-1 Cells Are Activated to Migrate to Lymphoid Organs Where within 1 Day They Produce IgM Antibodies That Initiate Elicitation of Contact Sensitivity
The Journal of ImmunologyItakura, A., Szczepanik, M., Campos, R.A., Paliwal, V., Majewska, M., Matsuda, H., Takatsu, K., Askenase, P.W.
2005
Elicitation of contact sensitivity (CS), a classic example of T cell-mediated immunity, requires Ag-specific IgM Abs to trigger an initiation process. This early process leads to local recruitment of CS-effector T cells after secondary Ag challenge. These Abs are produced by the B-1 subset of B cells within 1 day after primary skin immunization. In this study we report the surprising observation that B-1 cells in the peritoneal cavity are activated as early as 1 h after naive mice are painted with a contact-sensitizing Ag on the skin of the trunk and feet to begin the initiation of CS. B-1 cells in the spleen and draining lymph nodes produce the initiating Abs by 1 day after immunization, when we found increased numbers of Ag-specific IgM Ab-producing cells in these tissues by ELISPOT assay. Importantly, we show that contact-activated peritoneal B-1 cells migrate to these lymphoid tissues and then differentiate into Ag-specific IgM Ab-producing cells, resulting in specific CS-initiating IgM Abs in the serum by 1 day. Furthermore, pertussis toxin, which is known to inhibit signaling via G protein-coupled chemokines, inhibited the migration of contact-activated peritoneal B-1 cells to the lymphoid tissues, probably due to BLR-1 (Burkitt lymphoma receptor-1). These findings indicate that within 1 h after contact skin immunization, B-1 cells in the peritoneal cavity are activated to migrate to the lymphoid tissues by chemokine-dependent mechanisms to produce serum Ag-specific IgM Abs within 1 day after immunization, leading to local recruitment of CS-effector T cells.
Development of a directed beam monochromatic UV light device for investigation of DNA damage in skin cancer
American Society for Biochemistry & Molecular BiologyVipin Paliwal and Nazieh Masoud
April 5, 2019
We developed a small portable UVB light source with directed beam emitting single wavelength at 308 nm. A mixture of Xe and Cl was used to form the XeCl* excimer which specifically emits the wavelength of 308 nm. The source is completely sealed and portable and can be transported to any laboratory, classroom or clinic. The source generates UVB light inside a ¼ inch diameter sealed quartz tube such that the light leaves from one end of the tube (directed beam) which can be regulated in intensity. The utility of the UVB source was tested by studying quantitative DNA damage (6-4 photoproducts) in calf thymus DNA. We conclude that the formation of 6-4 pyrimidine-pyrimidone photoproducts is proportional to the dose of UVB radiation.
